6QNZ
Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Heterodimeric restriction endonuclease R.BspD6I large subunit | polymer | 604 | 70815.1 | 2 | UniProt (A3FEV7) Pfam (PF09491) | Bacillus sp. D6 | |
| 2 | C, D (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 3 | E, F, G, H, I (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 5 | Chemie (PO4) | |||
| 4 | J, K (A, B) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 604 (UniProt: A3FEV7)
| PDB | External Database | Details |
|---|---|---|
| Ala 418 | Glu 418 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 141630.3 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 659.0 | |
| All* | Total formula weight | 142289.3 |
