2EWF

Crystal structure of the site-specific DNA nickase N.BspD6I

Summary for 2EWF

DescriptorNicking endonuclease N.BspD6I, BROMIDE ION (3 entities in total)
Functional Keywordshelix-turn-helix, beta-alpha-barrel, hydrolase
Biological sourceBacillus sp.
Total number of polymer chains1
Total molecular weight72740.83
Authors
Kachalova, G.S.,Bartunik, H.D.,Artyukh, R.I.,Rogulin, E.A.,Perevyazova, T.A.,Zheleznaya, L.A.,Matvienko, N.I. (deposition date: 2005-11-03, release date: 2006-11-21, Last modification date: 2011-07-13)
Primary citation
Kachalova, G.S.,Rogulin, E.A.,Yunusova, A.K.,Artyukh, R.I.,Perevyazova, T.A.,Matvienko, N.I.,Zheleznaya, L.A.,Bartunik, H.D.
Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit.
J.Mol.Biol., 384:489-502, 2008
PubMed: 18835275 (PDB entries with the same primary citation)
DOI: 10.1016/j.jmb.2008.09.033
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.84 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers180.7%8.1%2.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2ewf
no rotation
Molmil generated image of 2ewf
rotated about x axis by 90°
Molmil generated image of 2ewf
rotated about y axis by 90°