6QIP
Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-biniding side chain of the human growth hormone derivative somapacitan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.020, 114.450, 159.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.479 - 2.450 |
R-factor | 0.2182 |
Rwork | 0.216 |
R-free | 0.25850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k71 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.504 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.140 | 2.538 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.084 | 1.683 |
Rmeas | 0.091 | 1.831 |
Rpim | 0.035 | 0.718 |
Number of reflections | 52497 | 5168 |
<I/σ(I)> | 17.08 | 1.12 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 6.4 |
CC(1/2) | 0.999 | 0.585 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | sodium cacodylate, PEG3350 |