6Q9L
HDM2 (17-111, WILDTYPE) COMPLEXED WITH COMPOUND 9 AT 1.13A; Structural states of Hdm2 and HdmX: X-ray elucidation of adaptations and binding interactions for different chemical compound classes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97848 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.773, 87.407, 37.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.130 |
| R-factor | 0.1788 |
| Rwork | 0.178 |
| R-free | 0.18980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dij |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.170 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.065 | 0.178 |
| Number of reflections | 63421 | 6856 |
| <I/σ(I)> | 12.1 | 3.05 |
| Completeness [%] | 91.2 | 70.5 |
| Redundancy | 5.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.7M AmSO4, 0.2M NaI, 0.2M NaCl, 0.2M TRIS |
