6PZV
Crystal Structure of Bovine DNMT1 RFTS domain in complex with H3K9me3 and Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.322, 196.445, 67.703 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.110 - 3.010 |
R-factor | 0.2217 |
Rwork | 0.216 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.531 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 3.118 |
High resolution limit [Å] | 3.010 | 3.010 |
Rmerge | 0.126 | |
Number of reflections | 19128 | 1774 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 99.1 | |
Redundancy | 9.8 | |
CC(1/2) | 0.998 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 277 | 0.1M citric acid (pH 3.5), 28% PEG8000 |