6PW3

LARP1 DM15 FYRE (F844Y, R847E) mutant bound to m7GpppG dinucleotide (capG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X17B1
Synchrotron site	NSLS
Beamline	X17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 1 21 1
Unit cell lengths	58.582, 87.355, 72.890
Unit cell angles	90.00, 93.37, 90.00

Refinement procedure

Resolution	29.240 - 2.340
R-factor	0.2235
Rwork	0.221
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zc4
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	2.400
High resolution limit [Å]	2.340	2.340
Rmerge	0.050	0.580
Number of reflections	30894	2210
<I/σ(I)>	12.6
Completeness [%]	99.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM Hepes 7.5, 0.08 M NaCl, 36% PEG 3350