6PQ3
Crystal structure of GDP-bound KRAS with ten residues long internal tandem duplication in the switch II region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 |
Unit cell lengths | 82.900, 82.900, 41.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.450 - 1.750 |
R-factor | 0.1827 |
Rwork | 0.179 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4obe |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.450 | 41.450 | 1.800 |
High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
Rmerge | 0.049 | 0.020 | 0.821 |
Rmeas | 0.052 | 0.021 | 0.871 |
Total number of observations | 146354 | ||
Number of reflections | 16461 | 203 | 1219 |
<I/σ(I)> | 21.59 | 70.36 | 2.67 |
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 8.891 | 8.621 | 8.928 |
CC(1/2) | 1.000 | 1.000 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM lithium acetate and 2.2 M ammonium sulfate |