6PJV
Structure of Human Sonic Hedgehog in complex with Zinc and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 118.728, 118.728, 118.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.500 - 1.430 |
| R-factor | 0.1244 |
| Rwork | 0.124 |
| R-free | 0.13910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhh |
| Data reduction software | XDS (January 26, 2018) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.545 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rpim | 0.021 | 0.462 |
| Number of reflections | 51261 | 2488 |
| <I/σ(I)> | 20 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.6 | 8.9 |
| CC(1/2) | 0.999 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 24% (w/v) polyethylene glycol 8000, 0.10 M TRIS pH 7.0, and 0.24 M MgCl2 |
