6PHB

Pfs25 in complex with the human transmission blocking antibody 2530

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 31
Unit cell lengths	114.599, 114.599, 98.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.511 - 2.000
R-factor	0.1778
Rwork	0.177
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uqy
RMSD bond length	0.002
RMSD bond angle	1.147
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.106	0.512
Rpim	0.059	0.288
Number of reflections	96360	13044
<I/σ(I)>	9.5	2.2
Completeness [%]	98.8	98.2
Redundancy	4.1	4
CC(1/2)	0.997	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M lithium sulfate, 0.1 M Tris, pH 7.0, and 2.0 M ammonium sulfate