6P8H
Crystal structure of CDK4 in complex with CyclinD1 and P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 62.620, 67.980, 185.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.698 - 3.190 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w96 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.492 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.680 | 3.410 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.167 | |
| Rpim | 0.080 | 0.406 |
| Number of reflections | 13821 | 2439 |
| <I/σ(I)> | 9.2 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.9 | 5.8 |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 100mM Tris (7.0), 10% PEG 8000 200mM MgCl2 |
