6P4B
HyHEL10 fab variant HyHEL10-4x (heavy chain mutations L4F, Y33H, S56N, and Y58F) bound to hen egg lysozyme variant HEL2x-flex (mutations R21Q, R73E, C76S, and C94S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.397, 65.722, 137.128 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 34.910 - 1.900 |
| R-factor | 0.2048 |
| Rwork | 0.203 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.623 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.250 | 37.250 | 1.840 |
| High resolution limit [Å] | 1.810 | 9.890 | 1.810 |
| Rmerge | 0.108 | 0.035 | 1.383 |
| Rmeas | 0.117 | 0.038 | 1.518 |
| Rpim | 0.045 | 0.015 | 0.619 |
| Total number of observations | 710161 | 4437 | 29197 |
| Number of reflections | 107151 | 697 | 5026 |
| <I/σ(I)> | 11 | 40.3 | 1.3 |
| Completeness [%] | 99.6 | 98 | 94.5 |
| Redundancy | 6.6 | 6.4 | 5.8 |
| CC(1/2) | 0.998 | 0.999 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM citrate, pH 4.2, 1 M lithium chloride, 11% w/v PEG3350 |
