6P4B

HyHEL10 fab variant HyHEL10-4x (heavy chain mutations L4F, Y33H, S56N, and Y58F) bound to hen egg lysozyme variant HEL2x-flex (mutations R21Q, R73E, C76S, and C94S)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (L, A)	HyHEL10 Fab light chain	polymer	214	23552.9	2	UniProt (A0A0E4B213) Pfam (PF07686) Pfam (PF07654)	Mus musculus (Mouse)
2	B, E (H, B)	HyHEL10 Fab heavy chain	polymer	223	24347.9	2		Mus musculus (Mouse)
3	C, F (C, D)	Lysozyme C	polymer	129	14241.9	2	UniProt (P00698) Pfam (PF00062)	Gallus gallus (Chicken)	1,4-beta-N-acetylmuramidase C, Allergen Gal d IV
4	G, H, I, J, K... (L, C, B, D)	CHLORIDE ION	non-polymer		35.5	9	Chemie (CL)
5	P, Q, R, S, T... (L, H, C, A, B...)	water	water		18.0	457	Chemie (HOH)

Sequence modifications

L, A: 1 - 106 (PDB: 6P4B)
L, A: 107 - 214 (UniProt: A0A0E4B213)
C, D: 1 - 129 (UniProt: P00698)

PDB	External Database	Details
Gln 21	Arg 39	engineered mutation
Glu 73	Arg 91	engineered mutation
Ser 76	Cys 94	engineered mutation
Ser 94	Cys 112	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	124285.3
Non-Polymers*	Number of molecules	9
	Total formula weight	319.1
All*	Total formula weight	124604.4

*Water molecules are not included.