6P4B

HyHEL10 fab variant HyHEL10-4x (heavy chain mutations L4F, Y33H, S56N, and Y58F) bound to hen egg lysozyme variant HEL2x-flex (mutations R21Q, R73E, C76S, and C94S)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002250	biological_process	adaptive immune response
A	0002376	biological_process	immune system process
A	0005576	cellular_component	extracellular region
A	0005886	cellular_component	plasma membrane
A	0019814	cellular_component	immunoglobulin complex
C	0003796	molecular_function	lysozyme activity
C	0003824	molecular_function	catalytic activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0016231	molecular_function	beta-N-acetylglucosaminidase activity
C	0016787	molecular_function	hydrolase activity
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0016998	biological_process	cell wall macromolecule catabolic process
C	0031640	biological_process	killing of cells of another organism
C	0042742	biological_process	defense response to bacterium
C	0042802	molecular_function	identical protein binding
C	0050829	biological_process	defense response to Gram-negative bacterium
C	0050830	biological_process	defense response to Gram-positive bacterium
C	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
D	0003796	molecular_function	lysozyme activity
D	0003824	molecular_function	catalytic activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005737	cellular_component	cytoplasm
D	0005783	cellular_component	endoplasmic reticulum
D	0016231	molecular_function	beta-N-acetylglucosaminidase activity
D	0016787	molecular_function	hydrolase activity
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0016998	biological_process	cell wall macromolecule catabolic process
D	0031640	biological_process	killing of cells of another organism
D	0042742	biological_process	defense response to bacterium
D	0042802	molecular_function	identical protein binding
D	0050829	biological_process	defense response to Gram-negative bacterium
D	0050830	biological_process	defense response to Gram-positive bacterium
D	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
L	0002250	biological_process	adaptive immune response
L	0002376	biological_process	immune system process
L	0005576	cellular_component	extracellular region
L	0005886	cellular_component	plasma membrane
L	0019814	cellular_component	immunoglobulin complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue CL L 301

Chain	Residue
L	PRO59
L	SER60
L	ARG61

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site_id	AC2
Number of Residues	3
Details	binding site for residue CL L 302

Chain	Residue
L	LYS103
L	ASP165
L	TYR173

---

site_id	AC3
Number of Residues	1
Details	binding site for residue CL L 303

Chain	Residue
L	GLN124

---

site_id	AC4
Number of Residues	3
Details	binding site for residue CL L 304

Chain	Residue
L	THR180
L	LEU160
L	THR178

---

site_id	AC5
Number of Residues	3
Details	binding site for residue CL L 305

Chain	Residue
B	THR86
B	THR87
L	ASP1

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site_id	AC6
Number of Residues	2
Details	binding site for residue CL C 202

Chain	Residue
C	HIS15
C	ILE88

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site_id	AC7
Number of Residues	1
Details	binding site for residue CL B 301

Chain	Residue
B	THR116

---

site_id	AC8
Number of Residues	1
Details	binding site for residue CL D 201

Chain	Residue
D	ILE88

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Functional Information from PROSITE/UniProt

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH

Chain	Residue	Details
L	TYR192-HIS198

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE:

Chain	Residue	Details
C	GLU35
C	ASP52
D	GLU35
D	ASP52

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site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING:

Chain	Residue	Details
C	ASP101
D	ASP101

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
C	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	ASN46
C	ASP48
C	SER50
C	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
C	ASN59

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site_id	MCSA2
Number of Residues	6
Details	M-CSA 203

Chain

Residue

Details

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