6P4B
HyHEL10 fab variant HyHEL10-4x (heavy chain mutations L4F, Y33H, S56N, and Y58F) bound to hen egg lysozyme variant HEL2x-flex (mutations R21Q, R73E, C76S, and C94S)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005886 | cellular_component | plasma membrane |
| C | 0003796 | molecular_function | lysozyme activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005783 | cellular_component | endoplasmic reticulum |
| C | 0005794 | cellular_component | Golgi apparatus |
| C | 0016231 | molecular_function | beta-N-acetylglucosaminidase activity |
| C | 0016998 | biological_process | cell wall macromolecule catabolic process |
| C | 0031640 | biological_process | killing of cells of another organism |
| C | 0042742 | biological_process | defense response to bacterium |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0050829 | biological_process | defense response to Gram-negative bacterium |
| C | 0050830 | biological_process | defense response to Gram-positive bacterium |
| C | 0051672 | biological_process | obsolete catabolism by organism of cell wall peptidoglycan in other organism |
| D | 0003796 | molecular_function | lysozyme activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005576 | cellular_component | extracellular region |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005783 | cellular_component | endoplasmic reticulum |
| D | 0005794 | cellular_component | Golgi apparatus |
| D | 0016231 | molecular_function | beta-N-acetylglucosaminidase activity |
| D | 0016998 | biological_process | cell wall macromolecule catabolic process |
| D | 0031640 | biological_process | killing of cells of another organism |
| D | 0042742 | biological_process | defense response to bacterium |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0050829 | biological_process | defense response to Gram-negative bacterium |
| D | 0050830 | biological_process | defense response to Gram-positive bacterium |
| D | 0051672 | biological_process | obsolete catabolism by organism of cell wall peptidoglycan in other organism |
| L | 0005576 | cellular_component | extracellular region |
| L | 0005886 | cellular_component | plasma membrane |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | binding site for residue CL L 301 |
| Chain | Residue |
| L | PRO59 |
| L | SER60 |
| L | ARG61 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue CL L 302 |
| Chain | Residue |
| L | LYS103 |
| L | ASP165 |
| L | TYR173 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | binding site for residue CL L 303 |
| Chain | Residue |
| L | GLN124 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue CL L 304 |
| Chain | Residue |
| L | THR180 |
| L | LEU160 |
| L | THR178 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue CL L 305 |
| Chain | Residue |
| B | THR86 |
| B | THR87 |
| L | ASP1 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue CL C 202 |
| Chain | Residue |
| C | HIS15 |
| C | ILE88 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 301 |
| Chain | Residue |
| B | THR116 |
| site_id | AC8 |
| Number of Residues | 1 |
| Details | binding site for residue CL D 201 |
| Chain | Residue |
| D | ILE88 |
Functional Information from PROSITE/UniProt
| site_id | PS00290 |
| Number of Residues | 7 |
| Details | IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH |
| Chain | Residue | Details |
| L | TYR192-HIS198 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 256 |
| Details | Domain: {"description":"C-type lysozyme","evidences":[{"source":"PROSITE-ProRule","id":"PRU00680","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 203 |
| Chain | Residue | Details |
| C | GLU35 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| C | ASN46 | |
| C | ASP48 | |
| C | SER50 | |
| C | ASP52 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction |
| C | ASN59 |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 203 |
| Chain | Residue | Details |
