6P3A
Crystal Structure Analysis of TAF1 Bromodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.430, 92.430, 117.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.108 - 2.990 |
| R-factor | 0.2564 |
| Rwork | 0.255 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uv5 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.720 | 3.040 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmeas | 0.242 | 3.236 |
| Rpim | 0.070 | 0.868 |
| Total number of observations | 133276 | 7079 |
| Number of reflections | 10768 | 518 |
| <I/σ(I)> | 9.1 | 0.9 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 12.4 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG8000, Tris pH 8.0 |
