6OQX
Human Liver Receptor Homolog-1 bound to the agonist 5N and a fragment of the Tif2 coregulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2015-10-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.547, 46.547, 220.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.052 - 2.004 |
R-factor | 0.2076 |
Rwork | 0.206 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l11 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.504 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 17346 | 1689 |
<I/σ(I)> | 21.3 | |
Completeness [%] | 99.9 | |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Sodium acetate (pH 4.6), PEG4000, glycerol |