6OB0
Compound 2 bound structure of WT Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D produced in HEK293-F cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 153.430, 191.420, 97.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.150 - 2.810 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6oaz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BUSTER (2.11.7) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.100 | 119.720 | 2.820 |
| High resolution limit [Å] | 2.810 | 13.030 | 2.810 |
| Rmerge | 0.148 | 0.015 | |
| Rmeas | 0.162 | 0.016 | |
| Rpim | 0.064 | 0.610 | |
| Number of reflections | 70015 | 793 | 693 |
| <I/σ(I)> | 12 | 33.4 | 2.3 |
| Completeness [%] | 99.1 | 97.3 | 99.7 |
| Redundancy | 6.4 | ||
| CC(1/2) | 0.998 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.15M calcium acetate, 18% PEG3350 |
