6O60

Crystal structure of GGTase3-FBXL2-SKP1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	86.843, 99.318, 151.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.451 - 2.503
R-factor	0.1968
Rwork	0.194
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3DSS and 1FQV
RMSD bond length	0.008
RMSD bond angle	1.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.098	0.890
Number of reflections	41359
<I/σ(I)>	24.3	2.3
Completeness [%]	90.5	93.6
Redundancy	4.8	4.7
CC(1/2)	0.991	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH6.0, 20%-22% (v/v) PEG 400