6NQT

GalNac-T2 soaked with UDP-sugar

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9753
Spacegroup name	P 21 21 21
Unit cell lengths	116.575, 120.126, 247.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.700 - 3.050
R-factor	0.23408
Rwork	0.231
R-free	0.28596
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d0t
RMSD bond length	0.016
RMSD bond angle	2.060
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	3.210
High resolution limit [Å]	3.050	3.050
Rmerge	0.135	0.771
Rpim	0.090	0.521
Number of reflections	64645	9466
<I/σ(I)>	7.7
Completeness [%]	97.1	98.3
Redundancy	3	3
CC(1/2)	0.989	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG