6NIP
Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ1 in complex with ZIKV E glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 417.330, 69.300, 212.580 |
| Unit cell angles | 90.00, 112.96, 90.00 |
Refinement procedure
| Resolution | 14.987 - 4.160 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mtx 5ire |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rpim | 0.190 | 0.560 |
| Number of reflections | 32942 | 3339 |
| <I/σ(I)> | 3.7 | 1.04 |
| Completeness [%] | 75.6 | 77.5 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 0.06 M MgCl2, 0.1M Imidazole MES monohydrate (pH 6.5), 20% Ethylene glycol and 10% PEG 8,000 |
