6NHA

Crystal structure of SYNT001, a human FcRn blocking monoclonal antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	64.830, 77.664, 215.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.769 - 2.381
R-factor	0.193
Rwork	0.190
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sqo 5k59 4n0f
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000	200.000	2.540
High resolution limit [Å]	2.380	6.790	2.500
Rmerge	0.107	0.043	1.816
Rmeas	0.116	0.046	1.981
Rpim	0.044	0.018	0.777
Number of reflections	41525	2153	1656
<I/σ(I)>	7.3
Completeness [%]	92.5	99.4	85.5
Redundancy	6.4	6.5	6
CC(1/2)		0.999	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277.15	0.1M CHES pH 9.5 50% polyethylene glycol 200