6NAJ
Integrin AlphaVBeta3 ectodomain bound to Hr10 variant of the 10th domain of Fibronectin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 129.713, 129.713, 308.198 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.620 - 3.100 |
| R-factor | 0.2394 |
| Rwork | 0.238 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mmz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.622 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 9.700 | |
| Number of reflections | 55225 | 5959 |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 97.0 | |
| Redundancy | 8.2 | |
| CC(1/2) | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 100 M Sodium Acetate, PEG4000, 800 mM Sodium Chloride. |
