6MPF

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a 2-thiocytidine (s2C32) and inosine (I34) modified anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 1 (TRNAARG1) bound to an mRNA with an CGC-codon in the A-site and paromomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	277
Detector technology	CCD
Collection date	2010-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	402.330, 402.330, 176.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.563 - 3.330
R-factor	0.1781
Rwork	0.178
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xnr
RMSD bond length	0.010
RMSD bond angle	1.602
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.563	3.449
High resolution limit [Å]	3.330	3.330
Number of reflections	375418	20244
<I/σ(I)>	12.98	1.42
Completeness [%]	97.7	97.82
Redundancy	3.1	3
CC(1/2)		0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5 VAPOR DIFFUSION, HANGING DROP, temperature 277K