6MPF

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a 2-thiocytidine (s2C32) and inosine (I34) modified anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 1 (TRNAARG1) bound to an mRNA with an CGC-codon in the A-site and paromomycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	16S rRNA	polymer	1508	489636.9	1		Thermus thermophilus HB8 (strain HB8 / ATCC 27634 / DSM 579)
2	B	30S ribosomal protein S2	polymer	234	26987.3	1	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
3	C	30S ribosomal protein S3	polymer	206	22862.4	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
4	D	30S ribosomal protein S4	polymer	208	24242.3	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
5	E	30S ribosomal protein S5	polymer	150	16331.1	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
6	F	30S ribosomal protein S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TS9
7	G	30S ribosomal protein S7	polymer	155	17919.8	1	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
8	H	30S ribosomal protein S8	polymer	138	15868.6	1	UniProt (P0DOY9) Pfam (PF00410) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
9	I	30S ribosomal protein S9	polymer	127	14279.4	1	UniProt (P80374) Pfam (PF00380) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
10	J	30S ribosomal protein S10	polymer	98	11299.2	1	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
11	K	30S ribosomal protein S11	polymer	119	12606.4	1	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
12	L	30S ribosomal protein S12	polymer	124	13804.3	1	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
13	M	30S ribosomal protein S13	polymer	125	14207.7	1	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
14	N	30S ribosomal protein S14 type Z	polymer	60	7027.5	1	UniProt (P0DOY6) Pfam (PF00253) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
15	O	30S ribosomal protein S15	polymer	88	10447.2	1	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
16	P	30S ribosomal protein S16	polymer	83	9924.5	1	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
17	Q	30S ribosomal protein S17	polymer	104	12194.5	1	UniProt (P0DOY7) Pfam (PF00366) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
18	R	30S ribosomal protein S18	polymer	73	8497.2	1	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
19	S	30S ribosomal protein S19	polymer	80	9203.7	1	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
20	T	30S ribosomal protein S20	polymer	99	10921.1	1	UniProt (P80380) Pfam (PF01649) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
21	V	30S ribosomal protein Thx	polymer	24	2960.5	1	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	S31
22	W	mRNA A-site fragment	polymer	4	1239.8	1		Escherichia coli
23	X	tRNA ASL Escherichia coli Arg1	polymer	11	3522.2	1		Escherichia coli
24	A	PAROMOMYCIN	non-polymer		615.6	1	Chemie (PAR)
25	A, E, J, W, X	MAGNESIUM ION	non-polymer		24.3	103	Chemie (MG)
26	D, N	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	767972.2
Non-Polymers*	Number of molecules	106
	Total formula weight	3249.9
All*	Total formula weight	771222.1

*Water molecules are not included.