6MKN
Structure of the Thermus thermophilus 30S ribosomal subunit complexed with an inosine (I34) modified anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 2 (TRNAARG2) bound to an mRNA with an CGU-codon in the A-site and paromomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2010-08-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 403.730, 403.730, 177.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.804 - 3.460 |
R-factor | 0.2031 |
Rwork | 0.203 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnr |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.8.4_1496) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.804 | 3.580 |
High resolution limit [Å] | 3.460 | 3.460 |
Rmerge | 0.069 | 0.866 |
Number of reflections | 318813 | 18061 |
<I/σ(I)> | 12.18 | |
Completeness [%] | 94.4 | 96.77 |
Redundancy | 3 | |
CC(1/2) | 0.983 | 0.581 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5 VAPOR DIFFUSION, HANGING DROP, temperature 277K |