6MKN

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with an inosine (I34) modified anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 2 (TRNAARG2) bound to an mRNA with an CGU-codon in the A-site and paromomycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	16S rRNA	polymer	1507	489191.6	1	GenBank (55771382)	Thermus thermophilus HB8
2	B	30S ribosomal protein S2	polymer	256	29317.7	1	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus HB8
3	C	30S ribosomal protein S3	polymer	239	26751.1	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus HB8
4	D	30S ribosomal protein S4	polymer	209	24373.4	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus HB8
5	E	30S ribosomal protein S5	polymer	162	17583.4	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus HB8
6	F	30S ribosomal protein S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus HB8	TS9
7	G	30S ribosomal protein S7	polymer	156	18051.0	1	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus HB8
8	H	30S ribosomal protein S8	polymer	138	15868.6	1	UniProt (P0DOY9) Pfam (PF00410) In PDB	Thermus thermophilus HB8
9	I	30S ribosomal protein S9	polymer	128	14429.7	1	UniProt (P80374) Pfam (PF00380) In PDB	Thermus thermophilus HB8
10	J	30S ribosomal protein S10	polymer	105	11955.0	1	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus HB8
11	K	30S ribosomal protein S11	polymer	129	13737.9	1	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus HB8
12	L	30S ribosomal protein S12	polymer	132	14637.4	1	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus HB8
13	M	30S ribosomal protein S13	polymer	126	14338.9	1	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus HB8
14	N	30S ribosomal protein S14 type Z	polymer	61	7158.7	1	UniProt (P0DOY6) Pfam (PF00253) In PDB	Thermus thermophilus HB8
15	O	30S ribosomal protein S15	polymer	89	10578.4	1	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus HB8
16	P	30S ribosomal protein S16	polymer	88	10410.0	1	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus HB8
17	Q	30S ribosomal protein S17	polymer	105	12324.7	1	UniProt (P0DOY7) Pfam (PF00366) In PDB	Thermus thermophilus HB8
18	R	30S ribosomal protein S18	polymer	88	10244.3	1	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	Thermus thermophilus HB8
19	S	30S ribosomal protein S19	polymer	93	10605.5	1	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus HB8
20	T	30S ribosomal protein S20	polymer	106	11736.1	1	UniProt (P80380) Pfam (PF01649) In PDB	Thermus thermophilus HB8
21	V	30S ribosomal protein Thx	polymer	27	3350.0	1	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus HB8	S31
22	X	tRNA ASL Escherichia coli Arg2	polymer	17	5442.3	1		Escherichia coli
23	W	mRNA A-site fragment	polymer	6	1899.2	1		Escherichia coli
24	A	PAROMOMYCIN	non-polymer		615.6	1	Chemie (PAR)
25	A, E, J	MAGNESIUM ION	non-polymer		24.3	78	Chemie (MG)

Sequence modifications

A: 5 - 1539 (GenBank: 55771382)

PDB	External Database	Details
-	G 132279	deletion
-	G 132309	deletion
-	C 132310	deletion
-	G 132311	deletion
-	A 132312	deletion
-	C 132643	deletion
U 1540	-	insertion
U 1541	-	insertion
U 1542	-	insertion
C 1543	-	insertion
U 1544	-	insertion

I: 1 - 128 (UniProt: P80374)

PDB	External Database	Details
Arg 58	His 58	conflict

Q: 1 - 105 (UniProt: P0DOY7)

PDB	External Database	Details
Gln 96	Glu 96	conflict

R: 1 - 88 (UniProt: Q5SLQ0)

PDB	External Database	Details
Gly 60	Ala 60	conflict

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	785973.5
Non-Polymers*	Number of molecules	79
	Total formula weight	2511.4
All*	Total formula weight	788484.9

*Water molecules are not included.