6MCQ

L. pneumophila effector kinase LegK7 in complex with human MOB1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	107.400, 113.100, 157.850
Unit cell angles	90.00, 106.00, 90.00

Refinement procedure

Resolution	49.493 - 2.570
R-factor	0.2009
Rwork	0.199
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.493	49.493	2.640
High resolution limit [Å]	2.570	11.490	2.570
Rmerge	0.103	0.040	0.874
Rmeas	0.115	0.045	0.980
Total number of observations	292177
Number of reflections	57771	673	4248
<I/σ(I)>	9.72	27.15	1.82
Completeness [%]	99.8	98	99.8
Redundancy	5.058	5.14	4.93
CC(1/2)	0.998	0.998	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	0.1 M HEPES, pH 7.5, 23% PEG600, 0.1 M lithium chloride, 20 mM succinate, pH 7.0