6MAJ
HBO1 is required for the maintenance of leukaemia stem cells
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.95365 |
Spacegroup name | H 3 |
Unit cell lengths | 111.543, 111.543, 73.702 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.595 - 2.139 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23740 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.733 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.600 | 2.200 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.198 | 1.292 |
Rpim | 0.095 | 0.614 |
Number of reflections | 18887 | 1486 |
<I/σ(I)> | 5.7 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.3 | 5.3 |
CC(1/2) | 0.987 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystals were grown at 20 C in sitting drop plates in drops of 200 nL crystallant and 200 nL protein. The protein was at 2.5 mg/mL and the reservoir was 20% PEG 3350 with 244 mM diammonium tartrate. |