6M11
Crystal structure of Rnase L in complex with Sunitinib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2018-09-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.240, 111.000, 262.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.260 - 2.460 |
R-factor | 0.2234 |
Rwork | 0.220 |
R-free | 0.28520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o1p |
RMSD bond length | 0.013 |
RMSD bond angle | 1.851 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.260 | 2.548 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.023 | 0.323 |
Number of reflections | 63535 | 63535 |
<I/σ(I)> | 14.17 | |
Completeness [%] | 99.3 | |
Redundancy | 2.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG4000, 100 mM Tris, 150 mM (NH4)2SO4 and 2 mM DTT buffer, pH 7.5 |