6LB7

Crystal structure of the Ca2+-free and Ca2+-bound MICU1-MICU2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	85.481, 107.721, 170.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.300 - 2.101
R-factor	0.1947
Rwork	0.194
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nsc 6iih
RMSD bond length	0.021
RMSD bond angle	1.292
Data reduction software	HKL-2000 (v716.1)
Data scaling software	HKL-2000 (v716.1)
Phasing software	PHENIX (1.10.1-2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	2.176
High resolution limit [Å]	2.101	2.101
Rmerge	0.125	1.192
Rmeas	0.130
Number of reflections	92168	8058
<I/σ(I)>	10.87	1.35
Completeness [%]	98.0	100
Redundancy	13	12.3
CC(1/2)	0.984	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293.15	0.2 M ammonium chloride, 5% (w/v) polyethylene glycol (PEG) 3350