6KQQ
NSD1 SET domain in complex with BT3 and SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 |
Unit cell lengths | 40.469, 60.811, 64.255 |
Unit cell angles | 101.18, 107.76, 93.58 |
Refinement procedure
Resolution | 30.350 - 1.800 |
R-factor | 0.1651 |
Rwork | 0.163 |
R-free | 0.20120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.004 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.680 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 50805 | 2529 |
<I/σ(I)> | 17.42 | 2.36 |
Completeness [%] | 97.3 | 95.4 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 18% PEG 8000, 100mM sodium cacodylate, pH 6.5, 200mM calcium Acetate |