6KO5
Complex structure of Ghrelin receptor with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-01 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 253.760, 44.750, 94.400 |
Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
Resolution | 49.421 - 3.300 |
R-factor | 0.2131 |
Rwork | 0.210 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4s0v |
RMSD bond length | 0.002 |
RMSD bond angle | 0.505 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.421 | 49.421 | 3.500 |
High resolution limit [Å] | 3.300 | 9.270 | 3.300 |
Rmerge | 0.874 | 0.190 | 4.384 |
Rmeas | 0.887 | 0.193 | 4.445 |
Total number of observations | 592198 | ||
Number of reflections | 16374 | 794 | 2583 |
<I/σ(I)> | 10.26 | 41.7 | 1.7 |
Completeness [%] | 99.9 | 99.2 | 99.9 |
Redundancy | 36.167 | 35.244 | 36.694 |
CC(1/2) | 0.991 | 0.997 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100 mM MES (pH6.6-7.0), 400 mM potassium acetate and 36-40% polyethylene glycol (PEG) 300. |