6KI6
Crystal structure of BCL11A in complex with gamma-globin -115 HPFH region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.555, 59.555, 213.367 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.078 - 2.500 |
R-factor | 0.2088 |
Rwork | 0.207 |
R-free | 0.25140 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.083 | 0.056 | 0.994 |
Rmeas | 0.089 | 0.060 | 1.062 |
Rpim | 0.030 | 0.022 | 0.366 |
Total number of observations | 141021 | ||
Number of reflections | 16230 | 930 | 757 |
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.9 | 99.6 | 99.9 |
Redundancy | 8.7 | 7.9 | 7.8 |
CC(1/2) | 0.995 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate (pH6.5), 18% w/v polyethylene glycol 8,000 |