6KI6

Crystal structure of BCL11A in complex with gamma-globin -115 HPFH region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	59.555, 59.555, 213.367
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.078 - 2.500
R-factor	0.2088
Rwork	0.207
R-free	0.25140
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.083	0.056	0.994
Rmeas	0.089	0.060	1.062
Rpim	0.030	0.022	0.366
Total number of observations	141021
Number of reflections	16230	930	757
<I/σ(I)>	9.1
Completeness [%]	99.9	99.6	99.9
Redundancy	8.7	7.9	7.8
CC(1/2)		0.995	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate (pH6.5), 18% w/v polyethylene glycol 8,000