6KDB

Crystal structure of human DNMT3B-DNMT3L in complex with DNA containing CpGpT site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2018-04-11
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	194.827, 194.827, 49.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.615 - 2.862
R-factor	0.1641
Rwork	0.163
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.047	0.524
Number of reflections	48145	2592
<I/σ(I)>	29.05
Completeness [%]	99.2
Redundancy	4.5
CC(1/2)		0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	50 mM MES (pH 5.6), 200 mM KCl, 10 mM MgSO4, 10% PEG 400