6K0Y
Study of the interactions of a novel monoclonal antibody, mAb059c, with the hPD-1 receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-06 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.952, 102.590, 137.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 - 1.700 |
| R-factor | 0.17317 |
| Rwork | 0.171 |
| R-free | 0.21447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ggs |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.166 | 1.061 |
| Rmeas | 0.053 | 0.421 |
| Number of reflections | 62244 | 2532 |
| <I/σ(I)> | 30.2 | 4.7 |
| Completeness [%] | 98.2 | 80.2 |
| Redundancy | 10 | 7 |
| CC(1/2) | 0.994 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M NaCl , 0.1M Tris pH 8, 14% PEG 4K |
