6J65
Crystal structure of human HINT1 mutant complexing with AP4A II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 46.300, 46.573, 64.246 |
Unit cell angles | 98.06, 89.82, 118.25 |
Refinement procedure
Resolution | 32.460 - 1.420 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eqe |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.065 | 0.427 |
Number of reflections | 78869 | |
<I/σ(I)> | 20.4 | |
Completeness [%] | 89.6 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | HEPES pH 7.5, PEG3 350 |