6IWD

The PTP domain of human PTPN14 in a complex with the CR3 domain of HPV18 E7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2017-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	54.573, 169.153, 42.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.987 - 1.800
R-factor	0.1873
Rwork	0.186
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bzl
RMSD bond length	0.007
RMSD bond angle	0.774
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.087	0.328
Number of reflections	35719	1615
<I/σ(I)>	41.3
Completeness [%]	95.1
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris-HCl pH 8.0, 25% (w/v) polyethylene glycol 3350, 0.25 M lithium sulfate monohydrate, and 2% (v/v) tert-butanol