6IRZ
Crystal structure of the zebrafish cap-specific adenosine methyltransferase bound to SAH and m7G-capped RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.669, 83.165, 93.132 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.566 - 2.000 |
R-factor | 0.1823 |
Rwork | 0.181 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6irx |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.900 | 46.900 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.063 | 0.047 | 0.654 |
Rmeas | 0.069 | 0.051 | 0.706 |
Rpim | 0.026 | 0.020 | 0.265 |
Total number of observations | 259727 | ||
Number of reflections | 38329 | 508 | 2771 |
<I/σ(I)> | 16.1 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 6.8 | 6.1 | 7.1 |
CC(1/2) | 0.998 | 0.997 | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 13% PEG 3350, 0.1M potassium thiocyanate, 0.1M bis-Tris propane |