6IBL

ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND AGONIST FORMOTEROL AND NANOBODY Nb80

Replaces:  6H7K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.966
Spacegroup name	P 21 21 21
Unit cell lengths	116.650, 121.120, 129.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.320 - 2.700
R-factor	0.24135
Rwork	0.240
R-free	0.27659
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h6x 3p0g
RMSD bond length	0.006
RMSD bond angle	1.541
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.570	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	50611
<I/σ(I)>	6.6
Completeness [%]	99.0
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Hepes-NaOH pH7.5 and 21-24% PEG1500