6I7Q

Structure of pVHL-elongin B-elongin C (VCB) in complex with hydroxylated-HIF-2alpha (523-542) in the C2221 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97627
Spacegroup name	C 2 2 21
Unit cell lengths	81.827, 86.758, 136.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.527 - 1.798
R-factor	0.1896
Rwork	0.187
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lqb
RMSD bond length	0.018
RMSD bond angle	1.399
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.560	1.850
High resolution limit [Å]	1.798	1.798
Rmerge	0.087	1.042
Rmeas	0.097	1.166
Rpim	0.043	1.171
Number of reflections	45358	3301
<I/σ(I)>	11.9	2.3
Completeness [%]	100.0	100
Redundancy	9.7	9.8
CC(1/2)	0.998	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	0.1M Cacodyl-Na pH 5.8, 0.1M Mg formate, 50 mM Cystamine, 22 % PEG3350