6I5N
Crystal structure of SOCS2:Elongin C:Elongin B in complex with growth hormone receptor peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 113.177, 156.763, 57.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.042 - 1.980 |
R-factor | 0.1909 |
Rwork | 0.190 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.064 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 113.550 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.094 | |
Number of reflections | 73251 | |
<I/σ(I)> | 17.1 | |
Completeness [%] | 100.0 | |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | Sodium cacodylate, cobalt chloride, MES, ammonium sulphate |