6I1L
Crystal structure of FnCas12a in complex with a crRNA guide and ssDNA target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.800, 188.580, 284.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.140 - 2.980 |
R-factor | 0.2066 |
Rwork | 0.204 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nfv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.667 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.140 | 3.086 |
High resolution limit [Å] | 2.980 | 2.980 |
Number of reflections | 87503 | |
<I/σ(I)> | 18.87 | 2.22 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.2 | 27.6 |
CC(1/2) | 0.999 | 0.675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6 M trisodium citrate |