6I1L
Crystal structure of FnCas12a in complex with a crRNA guide and ssDNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | CRISPR-associated endonuclease Cas12a | polymer | 1301 | 152194.1 | 2 | UniProt (A0Q7Q2) | Francisella tularensis subsp. novicida (strain U112) | CRISPR-associated endonuclease Cpf1,FnCas12a,FnCpf1 |
| 2 | B, E (B, E) | crRNA (40-MER) | polymer | 40 | 12754.5 | 2 | synthetic construct | ||
| 3 | C, F (C, F) | ssDNA target strand | polymer | 20 | 6082.0 | 2 | synthetic construct | ||
| 4 | G, H, HA, I, J... (A, E, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 9 | Chemie (MG) | |||
| 5 | AA, BA, IA, JA, K... (D, E, A, B) | CITRIC ACID | non-polymer | 192.1 | 11 | Chemie (CIT) | |||
| 6 | CA, DA, EA, FA, GA... (D, E, A, B) | POTASSIUM ION | non-polymer | 39.1 | 12 | Chemie (K) | |||
| 7 | MA, NA, OA, PA, QA (A, B, C, D, E) | water | water | 18.0 | 95 | Chemie (HOH) |
Sequence modifications
A, D: 2 - 1300 (UniProt: A0Q7Q2)
| PDB | External Database | Details |
|---|---|---|
| Asn 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Gln 1006 | Glu 1006 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 342061.3 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 2801.3 | |
| All* | Total formula weight | 344862.6 |
