Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6I1L

Crystal structure of FnCas12a in complex with a crRNA guide and ssDNA target

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004530	molecular_function	deoxyribonuclease I activity
A	0006259	biological_process	DNA metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0051607	biological_process	defense response to virus
D	0003677	molecular_function	DNA binding
D	0003723	molecular_function	RNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004530	molecular_function	deoxyribonuclease I activity
D	0006259	biological_process	DNA metabolic process
D	0016787	molecular_function	hydrolase activity
D	0016829	molecular_function	lyase activity
D	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue MG A 1401

Chain	Residue
A	ASN602
A	THR604
A	ASP616
A	HOH1510
A	HOH1530
A	HOH1536

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 1402

Chain	Residue
A	HOH1526
A	HOH1535
A	HOH1540
A	HOH1501
A	HOH1503
A	HOH1521

site_id	AC3
Number of Residues	6
Details	binding site for residue MG A 1403

Chain	Residue
A	CYS70
A	HOH1538
A	HOH1541
A	HOH1542
A	HOH1544
A	HOH1545

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 1404

Chain	Residue
A	ARG800
A	HOH1502
A	HOH1523
B	A-3
B	HOH203
B	HOH205

site_id	AC5
Number of Residues	4
Details	binding site for residue CIT A 1405

Chain	Residue
A	TYR773
A	SER776
A	GLN780
A	LYS782

site_id	AC6
Number of Residues	4
Details	binding site for residue CIT A 1406

Chain	Residue
A	LEU13
A	SER14
A	GLU1062
B	A1

site_id	AC7
Number of Residues	5
Details	binding site for residue CIT A 1407

Chain	Residue
A	ALA1079
A	PHE1081
A	THR1082
A	SER1083
A	LYS1084

site_id	AC8
Number of Residues	2
Details	binding site for residue K A 1408

Chain	Residue
A	ILE586
A	GLN588

site_id	AC9
Number of Residues	2
Details	binding site for residue K A 1409

Chain	Residue
A	HIS990
B	A-1

site_id	AD1
Number of Residues	3
Details	binding site for residue K A 1410

Chain	Residue
A	ARG18
B	U-15
B	CIT102

site_id	AD2
Number of Residues	3
Details	binding site for residue K A 1411

Chain	Residue
A	LEU66
A	VAL69
A	TYR248

site_id	AD3
Number of Residues	1
Details	binding site for residue K A 1412

Chain	Residue
A	ASP261

site_id	AD4
Number of Residues	6
Details	binding site for residue MG B 101

Chain	Residue
A	HOH1529
B	HOH201
B	HOH204
B	HOH206
B	HOH208
B	HOH210

site_id	AD5
Number of Residues	4
Details	binding site for residue CIT B 102

Chain	Residue
A	ARG18
A	ARG833
A	K1410
B	U-14

site_id	AD6
Number of Residues	2
Details	binding site for residue CIT B 103

Chain	Residue
A	LYS852
B	A-18

site_id	AD7
Number of Residues	1
Details	binding site for residue K B 104

Chain	Residue
B	A-1

site_id	AD8
Number of Residues	6
Details	binding site for residue MG D 1401

Chain	Residue
D	ASN602
D	THR604
D	ASP616
D	HOH1510
D	HOH1519
D	HOH1522

site_id	AD9
Number of Residues	6
Details	binding site for residue MG D 1402

Chain	Residue
D	ARG800
D	HOH1507
D	HOH1515
E	A-3
E	HOH201
E	HOH204

site_id	AE1
Number of Residues	6
Details	binding site for residue MG D 1403

Chain	Residue
D	HOH1502
D	HOH1505
D	HOH1506
D	HOH1525
D	HOH1526
E	HOH210

site_id	AE2
Number of Residues	4
Details	binding site for residue CIT D 1404

Chain	Residue
D	TYR773
D	SER776
D	GLN780
D	LYS782

site_id	AE3
Number of Residues	4
Details	binding site for residue CIT D 1405

Chain	Residue
D	ARG18
D	ARG833
D	K1408
E	U-14

site_id	AE4
Number of Residues	5
Details	binding site for residue CIT D 1406

Chain	Residue
D	GLY174
D	TRP175
D	THR176
D	THR177
D	HOH1510

site_id	AE5
Number of Residues	2
Details	binding site for residue K D 1407

Chain	Residue
D	ARG43
D	SER171

site_id	AE6
Number of Residues	2
Details	binding site for residue K D 1408

Chain	Residue
D	HIS881
D	CIT1405

site_id	AE7
Number of Residues	1
Details	binding site for residue K D 1409

Chain	Residue
D	THR160

site_id	AE8
Number of Residues	2
Details	binding site for residue K D 1410

Chain	Residue
D	SER603
D	LYS635

site_id	AE9
Number of Residues	2
Details	binding site for residue K D 1411

Chain	Residue
D	GLU1033
E	CIT102

site_id	AF1
Number of Residues	6
Details	binding site for residue MG E 101

Chain	Residue
D	HOH1524
E	HOH202
E	HOH203
E	HOH206
E	HOH207
E	HOH211

site_id	AF2
Number of Residues	4
Details	binding site for residue CIT E 102

Chain	Residue
D	LEU13
D	SER14
D	K1411
E	A1

site_id	AF3
Number of Residues	3
Details	binding site for residue CIT E 103

Chain	Residue
D	GLU1033
E	A1
E	G2

site_id	AF4
Number of Residues	4
Details	binding site for residue CIT E 104

Chain	Residue
D	ASN683
D	HIS843
D	LYS852
E	A-18

site_id	AF5
Number of Residues	2
Details	binding site for residue K E 105

Chain	Residue
E	C-13
E	U-14

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	628
Details	Region: {"description":"Recognition domain 1","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Region: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	8
Details	Region: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	6
Details	Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	6
Details	Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	16
Details	Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	140
Details	Region: {"description":"Wedge region 2","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	34
Details	Region: {"description":"LKL, important for PAM recognition and DNA unwinding","evidences":[{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	198
Details	Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	12
Details	Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	24
Details	Region: {"description":"Binds single-strand non-target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	20
Details	Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	60
Details	Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	34
Details	Region: {"description":"Bridge helix","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	6
Details	Active site: {"description":"For pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	6
Details	Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"26422227","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	2
Details	Site: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	2
Details	Site: {"description":"Binds target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	2
Details	Site: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	6
Details	Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	2
Details	Site: {"description":"Caps the crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	2
Details	Site: {"description":"Binds DNA protospacer adjacent motif (PAM)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	2
Details	Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	2
Details	Site: {"description":"Binds PAM","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	4
Details	Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	2
Details	Site: {"description":"Binds Target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	2
Details	Site: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	2
Details	Site: {"description":"Stabilizes transition state for pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI29
Number of Residues	4
Details	Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI30
Number of Residues	129
Details	Region: {"description":"Wedge region 3","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)