6HWE
Yeast 20S proteasome beta2-G45A mutant in complex with carfilzomib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 136.780, 300.170, 145.490 |
Unit cell angles | 90.00, 113.29, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.20566 |
Rwork | 0.204 |
R-free | 0.23057 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cz4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.053 | 0.548 |
Number of reflections | 467751 | |
<I/σ(I)> | 15.2 | 2.3 |
Completeness [%] | 98.4 | 99.1 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |