6HWE

Yeast 20S proteasome beta2-G45A mutant in complex with carfilzomib

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, O	Proteasome subunit alpha type-2	polymer	250	27191.8	2	UniProt (P23639) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit Y7,Multicatalytic endopeptidase complex subunit Y7,Proteasome component Y7,Proteinase YSCE subunit 7
2	B, P	Proteasome subunit alpha type-3	polymer	258	28748.2	2	UniProt (P23638) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit Y13,Multicatalytic endopeptidase complex subunit Y13,Proteasome component Y13,Proteinase YSCE subunit 13
3	C, Q	Proteasome subunit alpha type-4	polymer	254	28478.1	2	UniProt (P40303) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PRE6,Multicatalytic endopeptidase complex subunit PRE6,Proteasome component PRE6,Proteinase YSCE subunit PRE6
4	D, R	Proteasome subunit alpha type-5	polymer	260	28649.1	2	UniProt (P32379) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PUP2,Multicatalytic endopeptidase complex subunit PUP2,Proteasome component PUP2,Proteinase YSCE subunit PUP2
5	E, S	Proteasome subunit alpha type-6	polymer	234	25634.0	2	UniProt (P40302) Pfam (PF00227) Pfam (PF10584) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PRE5,Multicatalytic endopeptidase complex subunit PRE5,Proteasome component PRE5,Proteinase YSCE subunit PRE5
6	F, T	Probable proteasome subunit alpha type-7	polymer	288	31575.1	2	UniProt (P21242) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit C1,Multicatalytic endopeptidase complex subunit C1,Proteasome component C1,Proteinase YSCE subunit 1
7	G, U	Proteasome subunit alpha type-1	polymer	252	28033.8	2	UniProt (P21243) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit C7-alpha,Multicatalytic endopeptidase complex C7,Proteasome component C7-alpha,Proteasome component Y8,Proteinase YSCE subunit 7,SCL1 suppressor protein
8	H, V	Proteasome subunit beta type-2	polymer	232	25128.5	2	UniProt (P25043) Pfam (PF00227) Pfam (PF12465) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PUP1,Multicatalytic endopeptidase complex subunit PUP1,Proteasome component PUP1,Proteinase YSCE subunit PUP1
9	I, W	Proteasome subunit beta type-3	polymer	205	22627.8	2	UniProt (P25451) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PUP3,Multicatalytic endopeptidase complex subunit PUP3,Proteasome component PUP3
10	J, X	Proteasome subunit beta type-4	polymer	198	22545.7	2	UniProt (P22141) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit C11,Multicatalytic endopeptidase complex subunit C11,Proteasome component C11,Proteinase YSCE subunit 11
11	K, Y	Proteasome subunit beta type-5	polymer	212	23325.2	2	UniProt (P30656) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PRE2,Multicatalytic endopeptidase complex subunit PRE2,Proteasome component PRE2,Proteinase YSCE subunit PRE2
12	L, Z	Proteasome subunit beta type-6	polymer	222	24883.9	2	UniProt (P23724) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Multicatalytic endopeptidase complex subunit C5,Proteasome component C5
13	M, a	Proteasome subunit beta type-7	polymer	246	27200.9	2	UniProt (P30657) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PRE4,Multicatalytic endopeptidase complex subunit PRE4,Proteasome component PRE4,Proteinase YSCE subunit PRE4
14	N, b	Proteasome subunit beta type-1	polymer	196	21517.2	2	UniProt (P38624) Pfam (PF00227) In PDB	Saccharomyces cerevisiae S288C	Macropain subunit PRE3,Multicatalytic endopeptidase complex subunit PRE3,Proteasome component PRE3,Proteinase YSCE subunit PRE3
15	G, I, K, L, N...	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
16	G, U	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
17	H, K, N, V, Y...	(2~{S})-~{N}-[(2~{S})-1-[[(3~{R},4~{S})-2,6-dimethyl-2,3-bis(oxidanyl)heptan-4-yl]amino]-1-oxidanylidene-3-phenyl-propan-2-yl]-4-methyl-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)-4-phenyl-butanoyl]amino]pentanamide	non-polymer		723.9	6	Chemie (GWZ)
18	H, K, V, Y	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	4	Chemie (MES)
19	N, b	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
20		water	water		18.0	2257	Chemie (HOH)

Sequence modifications

H, V: 1 - 232 (UniProt: P25043)

PDB	External Database	Details
Ala 45	Gly 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	731078.8
Non-Polymers*	Number of molecules	22
	Total formula weight	5582.1
All*	Total formula weight	736660.9

*Water molecules are not included.