6HPR

Crystal structure of cIAP1 RING domain bound to UbcH5B-Ub and a non-covalent Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	79.190, 53.600, 78.540
Unit cell angles	90.00, 107.57, 90.00

Refinement procedure

Resolution	23.515 - 1.700
R-factor	0.1718
Rwork	0.170
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eb6 3zni
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.520	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.063	0.539
Rmeas	0.075
Rpim	0.040
Number of reflections	34206
<I/σ(I)>	13.8	2
Completeness [%]	98.8	94.7
Redundancy	3.3	2.7
CC(1/2)	0.998	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M ammonium fluoride and 15% (w/v) PEG 3350