6HPH
Crystal structure of human Pif1 helicase in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.280, 143.290, 77.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.950 - 1.130 |
| R-factor | 0.12881 |
| Rwork | 0.127 |
| R-free | 0.16304 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hpq |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.496 |
| Data reduction software | xia2 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.950 | 1.160 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.068 | 0.800 |
| Number of reflections | 149927 | 9913 |
| <I/σ(I)> | 11.2 | 1.4 |
| Completeness [%] | 98.7 | 89.63 |
| Redundancy | 6.3 | 4 |
| CC(1/2) | 0.997 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Na-acetate, PEG 8K, PEG1K |
