6HPH
Crystal structure of human Pif1 helicase in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.280, 143.290, 77.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.950 - 1.130 |
R-factor | 0.12881 |
Rwork | 0.127 |
R-free | 0.16304 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hpq |
RMSD bond length | 0.029 |
RMSD bond angle | 2.496 |
Data reduction software | xia2 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.950 | 1.160 |
High resolution limit [Å] | 1.130 | 1.130 |
Rmerge | 0.068 | 0.800 |
Number of reflections | 149927 | 9913 |
<I/σ(I)> | 11.2 | 1.4 |
Completeness [%] | 98.7 | 89.63 |
Redundancy | 6.3 | 4 |
CC(1/2) | 0.997 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Na-acetate, PEG 8K, PEG1K |