6H8K

Crystal structure of a variant (Q133C in PSST) of Yarrowia lipolytica complex I

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	316.310, 316.310, 819.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.790
R-factor	0.361
Rwork	0.361
R-free	0.36300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wz7
RMSD bond length	0.009
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.990
High resolution limit [Å]	3.790	3.790
Rmerge	0.204
Number of reflections	120019
<I/σ(I)>	13.2	0.7
Completeness [%]	99.6
Redundancy	21
CC(1/2)	1.000	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	291	PEG 3350, calcium acetate, glycerol