6H0B
Crystal structure of the human GalNAc-T4 in complex with UDP, manganese and the diglycopeptide 6.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.090, 81.332, 84.863 |
| Unit cell angles | 65.55, 68.66, 74.31 |
Refinement procedure
| Resolution | 73.990 - 1.800 |
| R-factor | 0.19092 |
| Rwork | 0.190 |
| R-free | 0.23806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nqa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.990 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.044 | 0.742 |
| Number of reflections | 133764 | |
| <I/σ(I)> | 9.2 | 0.9 |
| Completeness [%] | 97.3 | 95.6 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.998 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG3350 ammonium nitrate |
