6GYR

Transcription factor dimerization activates the p300 acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.282
Spacegroup name	P 1 21 1
Unit cell lengths	100.826, 146.717, 116.477
Unit cell angles	90.00, 91.75, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.2113
Rwork	0.209
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhw
RMSD bond length	0.008
RMSD bond angle	1.242
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.250
High resolution limit [Å]	3.100	3.100
Number of reflections	106462
<I/σ(I)>	7.48
Completeness [%]	99.0
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	100 mM HEPES, pH 7.5, 18-22% polyethylene glycol 3350, 0.2 M NaCl